Coding
Part:BBa_K1146002
Designed by: Areum Goh Group: iGEM13_Korea_U_Seoul (2013-09-11)
YfaL_NacreinDR
It is DNA coding truncated autotransporter (YfaL) of E. coli and repeat-deleted nacrein. An usage of truncated autotransporter is displaying recombinant protein in the cell surface of E. coli. Nacrein has a domain for carbonic anhydrase (CA) and repeat sequence for binding calcuim ion. We deleted repeat sequence of nacrein and we called it as NacreinDR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2322
Illegal BamHI site found at 1593 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 984
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Categories
Parameters
None |