Coding

Part:BBa_K1146002

Designed by: Areum Goh   Group: iGEM13_Korea_U_Seoul   (2013-09-11)


YfaL_NacreinDR

It is DNA coding truncated autotransporter (YfaL) of E. coli and repeat-deleted nacrein. An usage of truncated autotransporter is displaying recombinant protein in the cell surface of E. coli. Nacrein has a domain for carbonic anhydrase (CA) and repeat sequence for binding calcuim ion. We deleted repeat sequence of nacrein and we called it as NacreinDR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2322
    Illegal BamHI site found at 1593
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 984


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Categories
Parameters
None